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Bugzilla – Bug 1173 |
Wrong metadata for Leica lif files |
Last modified: 2015-10-12 15:05:13 CDT |
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NOTICE! This is a static HTML version of a legacy Fiji BugZilla bug. The Fiji project now uses GitHub Issues for issue tracking. Please file all new issues there. |
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Created attachment 267 example lif file In case you cannot see the right metadata, here it is: Image: ppkR-mCard-40x CB z4 633 0.5p Size: 524.29 KB File Location: D:\02C盘\桌面\Experiment001.lif Start Time: 9/27/2015 12:06:23 AM.620 End Time: 9/27/2015 12:06:23 AM.620 Total Exposures: 2 (2 channels, 1 frames) Data from: LAS-AF 3.3.0.10134 ________________________________________ Dimensions Dimension Logical Size Physical Length Physical Origin Pixel Size / Voxel Size X 512 72.66 µm 0.00 µm 0.142 µm Y 512 72.66 µm 0.00 µm 0.142 µm Channels LUT Resolution Min Max STED: DetectorMode / Huygens saturation factor / Wavelength Red 8 0 255 --- / --- / --- Green 8 0 255 --- / --- / --- Time Stamps: Frame (Show All) Relative Time (s) Absolute Time (h:m:s.ms) Date 1 0 12:06:23 AM.620 9/27/2015 2 0 12:06:23 AM.620 9/27/2015 Frame (Show first + last) Relative Time Absolute Time Date 1 0 12:06:23 AM.620 9/27/2015 2 0 12:06:23 AM.620 9/27/2015 Confocal Settings Name Value Scan Mode xyz Scan Direction X Bidirectional Scan Speed 600 Hz Version Number 6 StagePosX 15205.0 µm StagePosY 7973.1 µm ZPosition 9.2 µm IsSuperZ 0 Magnification 40 ObjectiveName HC PL APO CS2 40x/1.30 OIL Immersion OIL NumericalAperture 1.30 RefractionIndex 1.518 Zoom 4.00 Pinhole 130.6 µm PinholeAiry 2.00 AU EmissionWavelength for PinholeAiry Calculation 580.0 nm FrameAverage 1 LineAverage 2 FrameAccumulation 1 Line_Accumulation 1 IsUserSettingNameSet 0 IsRoiScanEnable 0 Filter Wheels / Other Motorized Devices Device Name Filter Name/Position Excitation Beam Splitter TD 488/561/633 Galvo Slider Galvo X Normal Notch FW 2 Empty Polarization FW NF 488/561/633 Galvo Resonant Pan Galvo X Pan Center Simple Beam Expander No FRAP Booster Target Slider Target Park X2 Lens Changer CS2 UV Optics 1 Laser Lines Laser Line Intensity UV ( 405 nm) Shutter: off, Intensity: 0.0000% Visible ( 458 nm) Shutter: on, Intensity: 0.0000% Visible ( 488 nm) Shutter: on, Intensity: 0.0000% Visible ( 514 nm) Shutter: on, Intensity: 0.0000% Visible ( 561 nm) Shutter: on, Intensity: 0.1001% Visible ( 633 nm) Shutter: on, Intensity: 0.0000% Detectors Name Channel Type Location Active Gain Offset Gate Start Gate End Gate Ref. Wavelength PMT 1 Channel 1 PMT (380nm - 391nm) Internal Inactive 0.0 0.00 -- Time Gating not supported -- HyD 2 Channel 2 HyD (566nm - 601nm) Standard mode Internal Inactive 15.0 -0.01 -- Time Gating not supported -- PMT 3 Channel 3 PMT (621nm - 626nm) Internal Inactive 0.0 0.00 -- Time Gating not supported -- HyD 4 Channel 4 HyD (640nm - 700nm) Standard mode Internal Active 50.0 -0.01 -- Time Gating not supported -- PMT 5 Channel 5 PMT (737nm - 779nm) Internal Inactive 0.0 0.00 -- Time Gating not supported -- PMT Trans Transmission Channel PMT TLD Inactive 0.0 0.00 -- Time Gating not supported -- Lasers LaserName OutputPower 405 Diode Off Argon 0 % DPSS 561 On HeNe 633 On Sequential Setting Nr.1 PMT Inactive, Gain: 0, Offset: 0 HyD Active, Gain: 0, Offset: 0 PMT Inactive, Gain: 0, Offset: 0 HyD Inactive, Gain: 0, Offset: 0 PMT Inactive, Gain: 0, Offset: 0 PMT Inactive, Gain: 0, Offset: 0 Laser Line UV ( 405 nm) Intensity: 0.0000% Laser Line Visible ( 458 nm) Intensity: 0.0000% Laser Line Visible ( 488 nm) Intensity: 0.0000% Laser Line Visible ( 514 nm) Intensity: 0.0000% Laser Line Visible ( 561 nm) Intensity: 0.1001% Laser Line Visible ( 633 nm) Intensity: 0.0000% Sequential Setting Nr.2 PMT Inactive, Gain: 0, Offset: 0 HyD Inactive, Gain: 0, Offset: 0 PMT Inactive, Gain: 0, Offset: 0 HyD Active, Gain: 0, Offset: 0 PMT Inactive, Gain: 0, Offset: 0 PMT Inactive, Gain: 0, Offset: 0 Laser Line UV ( 405 nm) Intensity: 0.0000% Laser Line Visible ( 458 nm) Intensity: 0.0000% Laser Line Visible ( 488 nm) Intensity: 0.0000% Laser Line Visible ( 514 nm) Intensity: 0.0000% Laser Line Visible ( 561 nm) Intensity: 0.0000% Laser Line Visible ( 633 nm) Intensity: 0.4999%Created attachment 268 right metadata for the example lif file