Colocalization Threshold

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Colocalization Threshold (ImageJ - Fiji)
Author Tony Collins (and others?)
Maintainer Daniel White
File Colocalization.jar
Source in gitweb, modified from MBF
Latest version june 2009
Development status no longer actively supported, unlikely to be stable enough for real use. Use Coloc_2
Website MBF ImageJ manual


Sets the thresholds for colocalization analysis, and also calculates Manders coefficients, 2D histogram / scatter plot, and other stats.


Note: this plugin is now deprecated. You should use the newer Coloc 2 plugin instead.

See the great documentation for this plugin at MBF ImageJ manual page The method is that implemented in Costes et al. This uses a Pearson's correlation above and below the thresholds to iteratively find where the correlation between the two images is 0 and sets the thresholds there. It is pretty robust and importantly is reproducible and non subjective.

Use of this plugin should be followed by use of the Colocalization Test plugin, to see of the results are statistically significant and better then random overlap of the signal in the images.

See also the Colocalization Analysis tutorial, and the original documentation for this plugin on the MacBiophotonics site.