info from the imageJ: [Jpeg] Compression Type: Baseline [Jpeg] Data Precision: 8 bits [Jpeg] Image Height: 1024 pixels [Jpeg] Image Width: 1280 pixels [Jpeg] Number of Components: 3 [Jpeg] Component 1: Y component: Quantization table 0, Sampling factors 1 horiz/1 vert [Jpeg] Component 2: Cb component: Quantization table 0, Sampling factors 1 horiz/1 vert [Jpeg] Component 3: Cr component: Quantization table 0, Sampling factors 1 horiz/1 vert [JpegComment] Jpeg Comment: LEAD Technologies Inc. V1.01 [Jfif] Version: 1.0 [Jfif] Resolution Units: inch [Jfif] X Resolution: 0 dots [Jfif] Y Resolution: 0 dots ------------------------ Title: Captured FITC 1.jpg Width: 1280 pixels Height: 1024 pixels Pixel size: 1x1 pixel ID: -2 Coordinate origin: 0,0 Bits per pixel: 32 (RGB) No Threshold Uncalibrated Path: C:\Desktop\Captured FITC 1.jpg Screen location: 86,109 (1920x1200) No Overlay No Selection info from Fiji: SCIFIO version: 0.15.4 File format: JPEG --- Dataset Information --- BitsPerPixel = 8 PixelType = 1 Dataset name = C:\Users\m082752\Desktop\Captured FITC 1.jpg Image 0 Information Dimension order = X,Y,Channel Dimension lengths = 1280,1024,3 --- Global Metadata --- --- Image Metadata --- ------------------------ Title: Captured FITC 1.jpg Width: 1280 pixels Height: 1024 pixels Pixel size: 1x1 pixel^2 ID: -2 Coordinate origin: 0,0 Bits per pixel: 32 (RGB) No Threshold Uncalibrated Path: C:\Desktop\Captured FITC 1.jpg Screen location: 24,110 (1920x1200) No Overlay No Selection