P;ease provide a small test macro that reproduces the problem. The following macro, which opens three images and runs "Images to Stack", works as expected.

  run("Close All");
  run("Boats (356K)");
  run("Bridge (174K)");
  run("Dot Blot (7K)");
  run("Images to Stack", "method=[Copy (center)] name=Stack use");

I have no macro, please see attachments, thanks

Created attachment 230
image properties

Created attachment 231
error

Wayne is asking you to please create such a macro, which illustrates the problem you are experiencing. You can use the Macro Recorder to create one easily:

http://imagej.net/Macros#The_recorder

Based on your screenshots, I created a macro that reproduces the problem on my system:

newImage("5x7_000001.tif", "16-bit random", 2273, 3570, 4);
run("Properties...", "channels=1 slices=1 frames=4 unit=pixel pixel_width=1.0000 pixel_height=1.0000 voxel_depth=1.0000");
newImage("5x7_000002.tif", "16-bit random", 2273, 3181, 4);
run("Properties...", "channels=1 slices=1 frames=4 unit=pixel pixel_width=1.0000 pixel_height=1.0000 voxel_depth=1.0000");
run("Images to Stack");

The "Images to Stack" command does not work with stacks as input. Instead, use the Image>Stacks>Tools>Concatenate command,

Thanks Wayne!

Here is a patch that tells the user this when such a circumstance occurs:

diff --git a/src/main/java/ij/plugin/ImagesToStack.java b/src/main/java/ij/plugin/ImagesToStack.java
index e84d924..d72dc30 100644
--- a/src/main/java/ij/plugin/ImagesToStack.java
+++ b/src/main/java/ij/plugin/ImagesToStack.java
@@ -38,15 +38,22 @@ public class ImagesToStack implements PlugIn {
                        return;
                }

-               int count = 0;
+               int count = 0, stackCount = 0;
                image = new ImagePlus[wList.length];
                for (int i=0; i<wList.length; i++) {
                        ImagePlus imp = WindowManager.getImage(wList[i]);
                        if (imp.getStackSize()==1)
                                image[count++] = imp;
+                       else
+                               stackCount++;
                }
                if (count<2) {
-                       IJ.error("Images to Stack", "There must be at least two open images.");
+                       if (stackCount >= 2) {
+                               IJ.error("Images to Stack", "The \"Images to Stack\" command " +
+                                       "does not work with stacks as input.\nInstead, use the " +
+                                       "Image>Stacks>Tools>Concatenate command.");
+                       }
+                       else IJ.error("Images to Stack", "There must be at least two open images.");
                        return;
                }

Thanks Curtis. Your patch that provides a more informative error message is in the latest ImageJ daily build (1.49p8).

Created attachment 232
img properties

Created attachment 233
error

thank you all.

I understand that the "Images to stack" command does not work with multi frames images.  Upon transforming into multichannel images, the command still do not work.  Please see new attachments thanks

The "Images to Stack" command does not currently work with multi-channel images. You need to use the Image>Stack>Tools>Concatenate command.

Thanks Wayne.  

I cannot use "concatenate" because my images (about 100) have all slightly different dimensions (they come from grid stitching).

Afaik only "Images to stack" works with images of different sizes.

Best,

Run the following macro before using the Concatenate command. It zero pads all open images so that they have the same dimensions.

  maxWidth = 0;
  maxHeight = 0;
  for (i=1; i<=nImages; i++) {
     selectImage(i);
     if (getWidth>maxWidth) maxWidth=getWidth;
     if (getHeight>maxHeight) maxHeight=getHeight;
  }
  for (i=1; i<=nImages; i++) {
     selectImage(i);
     if (getWidth!=maxWidth || getHeight!=maxHeight)
        run("Canvas Size...", "width=&maxWidth height=&maxHeight position=Center zero");
  }

The Image>Stacks>Tools>Concatenate command works with images of different dimensions in the latest ImageJ daily build (1.49p9). You can upgrade by using the Help>Update ImageJ command and selecting "daily build" from the drop down menu.

Wayne, 
Thank you so much, this is great!
Best,