Bug 1140 – Blotting of lanes not working under Windows10

Bug 1140 - Blotting of lanes not working under Windows10


Summary:	Blotting of lanes not working under Windows10

Status:	RESOLVED WORKSFORME

Product:	Fiji
Classification:	Unclassified
Component:	Plugins
Version:	unspecified
Hardware:	PC Windows

Importance:	P4 normal
Assigned To:	tobias

URL:

Depends on:
Blocks:

Reported:	2015-08-13 04:58 CDT by tobias
Modified:	2015-08-14 05:48 CDT
CC List:	3 users (show)

See Also:

Attachments
screenshot showing steps of image analysis and blotting (291.64 KB, image/png) 2015-08-14 05:38 CDT, tobias

Description tobias 2015-08-13 04:58:31 CDT

Hi,

I was updating to Win10 and now realizing that I can't  plot lanes anymore when using the analyzing --> gel function.
Usually, I open the image, draw the rectangular around the band go on with Ctrl1 and Ctrl2 and finally blot with Ctrl3. This blotting does not work anymore. The same goes when I use these functions through the menus (Analyze --> gels and so on).
Hope, this bug can be fixd easily.

Best regards,

Tobias

Information about your version of Java:

  os.arch => amd64
  os.name => Windows NT (unknown)
  os.version => 6.2
  java.version => 1.6.0_24
  java.vendor => Sun Microsystems Inc.
  java.runtime.name => Java(TM) SE Runtime Environment
  java.runtime.version => 1.6.0_24-b07
  java.vm.name => Java HotSpot(TM) 64-Bit Server VM
  java.vm.version => 19.1-b02
  java.vm.vendor => Sun Microsystems Inc.
  java.vm.info => mixed mode
  java.awt.graphicsenv => sun.awt.Win32GraphicsEnvironment
  java.specification.name => Java Platform API Specification
  java.specification.version => 1.6
  sun.cpu.endian => little
  sun.desktop => windows
  file.separator => \

The up-to-date check says: REMIND_LATER

Information relevant to JAVA_HOME related problems:

  JAVA_HOME is set to: C:\Users\USER\DOCUME~1\Fiji.app/java/win64/jdk1.6.0_24//jre
  imagej.dir => C:\Users\USER\DOCUME~1\Fiji.app

Information about the version of each plugin:

Activated update sites:
ImageJ: http://update.imagej.net/ (last check:20150812152518)
Fiji: http://fiji.sc/update/ (last check:20150724195809)

Files not up-to-date:
  1ad3be0d (LOCAL_ONLY) 20141111135036 jars/jpedalSTD.jar
  404c9259 (LOCAL_ONLY) 20141215110731 plugins/ICTN_.jar

Comment 1 Wayne Rasband 2015-08-14 00:32:30 CDT

I cannot reproduce this problem using the Gel sample image. The commands in the Analyze>Gels sub-menu work as expected on Windows 10 using both Java 1.6.0_24 and Java 1.8.0_45.

Comment 2 tobias 2015-08-14 05:38:18 CDT

Created attachment 258
screenshot showing steps of image analysis and blotting

Dear Wayne,

I am glad to hear that it works for you under Win10. Unfortunately, it is not the case for me. I was attaching a screen shot showing the empty blot window.
Any idea?

Best regards,

Tobias

Comment 3 Jan Eglinger 2015-08-14 05:48:54 CDT

Hi Tobias,

as far as I can see from your attachment, you are measuring in the red channel (Channel 1), but your signal appear to be green, i.e. belongs to a different channel.
Please check the channel slider or split channels before measuring.

Hope that helps,
Jan