Consider the macro pasted below.
When running 'Image > Adjust > Size...' with bilinear or bicubic interpolation, the position of pixels gets shifted by 1.0 pixel in X, by 0.5 pixel in Y, and by 0.5 pixel in Z direction, when compared to scaling without interpolation.

With the macro below, I get the following values for the center of mass:

1	None:6	8	8	8	8
2	Bilinear:6	8	8	9	8.500
3	Bicubic:6	8	8	9	8.500

I would expect all values to be 8 in the above table. In addition, I see a shift in Z when scrolling through the three stacks side by side.


// macro code to illustrate scaling issue

newImage("Original", "8-bit black", 8, 8, 8);
setForegroundColor(255, 255, 255);
run("Specify...", "width=4 height=4 x=2 y=2 slice=3");
setSlice(3);
run("Fill", "slice");
setSlice(4);
run("Fill", "slice");
setSlice(5);
run("Fill", "slice");
setSlice(6);
run("Fill", "slice");
run("Select None");
run("Set... ", "zoom=1600 x=4 y=4");

run("Set Measurements...", "centroid center display redirect=None decimal=3");
run("Duplicate...", "title=None duplicate");
run("Size...", "width=16 height=16 depth=16 interpolation=None");
setSlice(6);
run("Set... ", "zoom=800 x=4 y=4");
run("Measure");

selectImage("Original");
run("Duplicate...", "title=Bilinear duplicate");
run("Size...", "width=16 height=16 depth=16 interpolation=Bilinear");
setSlice(6);
run("Set... ", "zoom=800 x=4 y=4");
run("Measure");

selectImage("Original");
run("Duplicate...", "title=Bicubic duplicate");
run("Size...", "width=16 height=16 depth=16 interpolation=Bicubic");
setSlice(6);
run("Set... ", "zoom=800 x=4 y=4");
run("Measure");

Information about your version of Java:

  os.arch => amd64
  os.name => Windows 7
  os.version => 6.1
  java.version => 1.6.0_24
  java.vendor => Sun Microsystems Inc.
  java.runtime.name => Java(TM) SE Runtime Environment
  java.runtime.version => 1.6.0_24-b07
  java.vm.name => Java HotSpot(TM) 64-Bit Server VM
  java.vm.version => 19.1-b02
  java.vm.vendor => Sun Microsystems Inc.
  java.vm.info => mixed mode
  java.awt.graphicsenv => sun.awt.Win32GraphicsEnvironment
  java.specification.name => Java Platform API Specification
  java.specification.version => 1.6
  sun.cpu.endian => little
  sun.desktop => windows
  file.separator => \

The up-to-date check says: REMIND_LATER

Information relevant to JAVA_HOME related problems:

  JAVA_HOME is set to: C:\UTILIT~1\FIJI-D~1.APP/java/win64/jdk1.6.0_24//jre
  imagej.dir => C:\UTILIT~1\FIJI-D~1.APP

Information about the version of each plugin:

Activated update sites:
ImageJ: http://update.imagej.net/ (last check:20151017033411)
Fiji: http://update.fiji.sc/ (last check:20151026112036)