Bugzilla – Bug 905 |
Threshold signed 16-bit images |
Last modified: 2014-08-15 14:21:20 CDT |
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Created attachment 199 Huygens Pro OME XML file This may be more than 1 issue. Source files are signed 16-bit OME XML from Huygens Professional. Opening by SCIFIO resets the histogram to 0-maximum, Bio-Formats displays the image as -32768 to 32767 until I hit 'Reset' in B&C. calling Threshold from menu or run("Threshold...") from macro will set by the default method. But, checking the box for 'Stack histogram' renders the channel black with min = 32768, max=32767 until hit 'reset' in threshold window or B&C window. When the channel is black, moving to a different channel and returning will restore the display but cancels the threshold. If I run setAutoThreshold("Default dark stack"); from the macro then it sets min = 32768, max=32767 and then all slices in the channel are masked as red. Smetimes If I click on 'Auto', this resets with the maybe the stack histogram. Other times I get min= -32768 and max= -max histogram (negative). Similar results with import by Bio-Formats, although it does not make the initial histogram reset. I'd like to call setAutoThreshold because I can specify the stack histogram. The run(Threshold) does not allow calling the stack histogram. I've attached a file from Huygens. Regards, glen